SheddomeDB 2023

UniProt Summary Literature Review Cleavage Sites Topological Domains Proteolytic Cleavage Secretome Detection

UniProt Summary
	UniProt ID	Q01705
	Entry Name	NOTC1_MOUSE
	Protein Name	Neurogenic locus notch homolog protein 1
	Primary Gene Name	Notch1
	Organism	Mus musculus (Mouse)
	Molecular Function	Activator Developmental protein Receptor
	Membrane Location	Cell membrane Membrane

Biological Literature Report of Shedding

	Substrate Name	Notch1
	Cell Type	Jurkat and C12D cells
	Substrate Description	cell fate and development
	Substrate Function Category	central nervous system (neuron)
	Substrate Function Subtype	Central nervous system (CNS) pathology; receptor
	Sheddase	ADAM17
	Shedding-related Disease	cell receptor
	Short Summary of Papers	ectodomain shedding result in RIP (regulated intramembrane proteolysis) and transcriptional activation of target genes
	Cleavage Information	NONE
	PMID	10882063 18951988

Cleavage Site Diagram

Topological Domains
Topological Domains	Start	End
Extracellular	19	1725
Transmembrane	1726	1746

Proteolytic Cleavage
Database	CutSeq	Mapped Position	Protease	Type	Evidence	PMID
MEROPS	RQRR*ELDP	1654	furin	physiologically relevant	NONE	NONE
MEROPS	KIEA*VKSE	1710	ADAM17 peptidase	physiologically relevant	NONE	10882063
MEROPS	YVAA*AAFV	1731	presenilin 1	physiologically relevant	NONE	12374741
MEROPS	VGCG*VLLS	1743	presenilin 1	physiologically relevant	NONE	12551931
MEROPS	VLPD*LSDQ	1823	granzyme B, rodent-type	not physiologically relevant	consensus sequence	16798735
MEROPS	VDAD*CMDV	1860	granzyme B, rodent-type	not physiologically relevant	consensus sequence	16798735
MEROPS	VISD*FIYQ	1902	granzyme B, rodent-type	not physiologically relevant	consensus sequence	16798735
MEROPS	VSAD*AQGV	1962	granzyme B, rodent-type	not physiologically relevant	consensus sequence	16798735
MEROPS	VLLD*HFAN	2072	granzyme B, rodent-type	not physiologically relevant	consensus sequence	16798735

Secretome Detection
Secretome Database	Cell Type or Body Fluid	PubMed Reference
SPD		22728825