SheddomeDB 2023

UniProt Summary Literature Review Cleavage Sites Topological Domains Proteolytic Cleavage Secretome Detection

UniProt Summary
	UniProt ID	P37840
	Entry Name	SYUA_HUMAN
	Protein Name	Alpha-synuclein
	Primary Gene Name	SNCA
	Organism	Homo sapiens (Human)
	Molecular Function
	Membrane Location	Secreted Membrane

Biological Literature Report of Shedding

	Substrate Name	α-synuclein
	Cell Type
	Substrate Description	May be involved in the regulation of dopamine release and transport.
	Substrate Function Category	disease
	Substrate Function Subtype
	Sheddase	MMP3,
	Shedding-related Disease	Parkinson disease (PD)
	Short Summary of Papers	MMP3 mediated the release α-synuclein proteolytic fragments which were sugggested to facilitate α-synuclein protein aggregation, the fragment-induced aggregates showed more toxic effect on cell viability.
	Cleavage Information	NONE
	PMID	15863497

Cleavage Site Diagram

Topological Domains
Topological Domains	Start	End
NO_INFO

Proteolytic Cleavage
Database	CutSeq	Mapped Position	Protease	Type	Evidence	PMID
MEROPS	M*DVFM	1	endopeptidase K	not physiologically relevant	NONE	19022250
MEROPS	MDVF*MKGL	4	matrix metallopeptidase-9	physiologically relevant	NONE	19022250
MEROPS	FMKG*LSKA	7	endopeptidase K	not physiologically relevant	NONE	19022250
MEROPS	FMKG*LSKA	7	matrix metallopeptidase-1	physiologically relevant	NONE	19022250
MEROPS	FMKG*LSKA	7	matrix metallopeptidase-9	physiologically relevant	NONE	19022250
MEROPS	KGLS*KAKE	9	calpain-1	physiologically relevant	N-terminal sequencing	17456777
PMAP	KGLS*KAKE	9	calpain-1	This_entry_is_based_on_an_experiment.	N-terminal sequencing	17456777
MEROPS	GLSK*AKEG	10	trypsin 1	not physiologically relevant	NONE	19022250
MEROPS	SKAK*EGVV	12	trypsin 1	not physiologically relevant	NONE	19022250
MEROPS	VVAA*AEKT	18	endopeptidase K	not physiologically relevant	NONE	19022250
MEROPS	VVAA*AEKT	18	matrix metallopeptidase-1	physiologically relevant	NONE	19022250
MEROPS	VVAA*AEKT	18	matrix metallopeptidase-9	physiologically relevant	NONE	19022250
MEROPS	AAEK*TKQG	21	matrix metallopeptidase-1	physiologically relevant	NONE	19022250
MEROPS	AAGK*TKEG	32	trypsin 1	not physiologically relevant	NONE	19022250
MEROPS	LYVG*SKTK	41	matrix metallopeptidase-1	physiologically relevant	NONE	19022250
MEROPS	VGSK*TKEG	43	trypsin 1	not physiologically relevant	NONE	19022250
MEROPS	VGSK*TKEG	43	endopeptidase K	not physiologically relevant	NONE	19022250
MEROPS	SKTK*EGVV	45	trypsin 1	not physiologically relevant	Mass spectroscopy	18500335
MEROPS	TKEG*VVHG	47	matrix metallopeptidase-1	physiologically relevant	NONE	19022250
MEROPS	HGVA*TVAE	53	endopeptidase K	not physiologically relevant	NONE	19022250
MEROPS	GVAT*VAEK	54	endopeptidase K	not physiologically relevant	NONE	19022250
MEROPS	GVAT*VAEK	54	matrix metallopeptidase-3	physiologically relevant	NONE	15863497
PMAP	GVAT*VAEK	54	matrix metallopeptidase-3	This_entry_is_based_on_an_experiment.	Mass spectrometry	15843175
MEROPS	TVAE*KTK	57	matrix metallopeptidase-3	physiologically relevant	NONE	15863497
MEROPS	VAEK*TKEQ	58	trypsin 1	not physiologically relevant	Mass spectroscopy	18500335
MEROPS	EKTK*EQVT	60	trypsin 1	not physiologically relevant	Mass spectroscopy	18500335
MEROPS	VTNV*GGAV	66	matrix metallopeptidase-9	physiologically relevant	NONE	19022250
MEROPS	GGAV*VTGV	70	endopeptidase K	not physiologically relevant	NONE	19022250
MEROPS	GGAV*VTGV	70	matrix metallopeptidase-1	physiologically relevant	NONE	19022250
MEROPS	GGAV*VTGV	70	matrix metallopeptidase-9	physiologically relevant	NONE	19022250
MEROPS	GAVV*TGVT	71	matrix metallopeptidase-1	physiologically relevant	NONE	19022250
MEROPS	GAVV*TGVT	71	matrix metallopeptidase-9	physiologically relevant	NONE	19022250
MEROPS	VVTG*VTAV	73	matrix metallopeptidase-9	physiologically relevant	NONE	19022250
MEROPS	VTGV*TAVA	74	matrix metallopeptidase-9	physiologically relevant	NONE	19022250
MEROPS	TAVA*QKTV	78	matrix metallopeptidase-1	physiologically relevant	NONE	19022250
MEROPS	TAVA*QKTV	78	matrix metallopeptidase-9	physiologically relevant	NONE	19022250
MEROPS	TAVA*QKTV	78	matrix metallopeptidase-3	physiologically relevant	NONE	15863497
PMAP	TAVA*QKTV	78	matrix metallopeptidase-3	This_entry_is_based_on_an_experiment.	Mass spectrometry	15843175 15863497
PMAP	TAVA*QKTV	78	matrix metallopeptidase-3	This_entry_is_based_on_an_experiment.	N-terminal sequencing	15863497
MEROPS	AVAQ*KTVE	79	matrix metallopeptidase-3	physiologically relevant	NONE	15863497
PMAP	AVAQ*KTVE	79	matrix metallopeptidase-3	This_entry_is_based_on_an_experiment.	N-terminal sequencing	15863497
PMAP	AVAQ*KTVE	79	matrix metallopeptidase-3	This_entry_is_based_on_an_experiment.	N-terminal sequencing	15863497
MEROPS	VAQK*TVEG	80	trypsin 1	not physiologically relevant	Mass spectroscopy	18500335
MEROPS	VAQK*TVEG	80	matrix metallopeptidase-3	physiologically relevant	NONE	19022250
MEROPS	GAGS*IAAA	87	matrix metallopeptidase-3	physiologically relevant	NONE	19022250
MEROPS	GSIA*AAT	89	endopeptidase K	not physiologically relevant	NONE	19022250
MEROPS	SIAA*ATG	90	matrix metallopeptidase-1	physiologically relevant	NONE	19022250
MEROPS	SIAA*ATG	90	matrix metallopeptidase-3	physiologically relevant	NONE	19022250
MEROPS	IAAA*TGF	91	endopeptidase K	not physiologically relevant	NONE	19022250
MEROPS	IAAA*TGF	91	matrix metallopeptidase-3	physiologically relevant	NONE	15863497
PMAP	IAAA*TGFV	91	matrix metallopeptidase-3	This_entry_is_based_on_an_experiment.	N-terminal sequencing	15863497
PMAP	IAAA*TGFV	91	matrix metallopeptidase-3	This_entry_is_based_on_an_experiment.	N-terminal sequencing	15863497
MEROPS	AAAT*GFVK	92	endopeptidase K	not physiologically relevant	NONE	19022250
MEROPS	AATG*FVKK	93	matrix metallopeptidase-3	physiologically relevant	NONE	15863497
PMAP	AATG*FVKK	93	matrix metallopeptidase-3	This_entry_is_based_on_an_experiment.	Mass spectrometry	15843175 15863497
PMAP	AATG*FVKK	93	matrix metallopeptidase-3	This_entry_is_based_on_an_experiment.	N-terminal sequencing	15863497
MEROPS	ATGF*VKKD	94	matrix metallopeptidase-3	physiologically relevant	NONE	19022250
MEROPS	FVKK*DQLG	97	trypsin 1	not physiologically relevant	NONE	19022250
MEROPS	FVKK*DQLG	97	matrix metallopeptidase-1	physiologically relevant	NONE	19022250
MEROPS	VKKD*QLGK	98	endopeptidase K	not physiologically relevant	NONE	19022250
MEROPS	VKKD*QLGK	98	matrix metallopeptidase-9	physiologically relevant	NONE	19022250
MEROPS	QLGK*NEEG	102	matrix metallopeptidase-3	physiologically relevant	NONE	15863497
MEROPS	DPDN*EAYE	122	calpain-1	physiologically relevant	NONE	17456777
PMAP	DPDN*EAYE	122	calpain-1	This_entry_is_based_on_an_experiment.	N-terminal sequencing	17456777
MEROPS	PDNE*AYEM	123	cathepsin D	physiologically relevant	N-terminal sequencing	18702517
MEROPS	EEGY*QDYE	133	matrix metallopeptidase-1	physiologically relevant	NONE	19022250
MEROPS	EGYQ*DYEP	134	matrix metallopeptidase-1	physiologically relevant	NONE	19022250

Secretome Detection
Secretome Database	Cell Type or Body Fluid	PubMed Reference
NO_DATA	NO_DATA	NO_DATA